Robust and flexible vaccination protocols using 6 different animal species

Overview of our recommendated animal immunization schedules and protocols for monoclonal antibody production: each protocol applies to antigen prime-boost into 2 animals (5 for mice), resulting in the output of serum, splenocytes or lymphocytes for 6 different animal species

Protocol	Prime-boost injections, and bleeds	PBMCs	Splenocytes or lymphocytes	Sera titer monitor
5 mice, 70 days	prime+1 boost, 2 bleeds	​✓	​✓	​✓
5 mice, 90 days	​prime+2 boost, 3 bleeds	​✓	​✓	​✓
2 rats, 80 days	​prime+2 boost, 3 bleeds	​✓	​✓	​✓
2  rhesus macaque, ​120 days	​prime+4 boost, 4 bleeds	​✓	Optional	​✓
2 rabbits, 70 days	prime+1 boost, 2 bleeds	​✓	Optional	​✓
2 rabbits, 90 days	​prime+2 boost, 3 bleeds	​✓	​Optional	​✓
2 guinea pigs, 90 days	prime+2 boost, 3 bleeds	​✓	​✓	​✓
2 sheep, 120 days	​prime+4 boost, 4 bleeds	​✓	Optional	​✓

By communication, immunization schedule and sample collection can be modified to benefit the projects. Antibodies from other animal species are possibly.

Suggestions for choosing the host species

Mouse: Primarily used for generating monoclonal antibodies. Due to limited volume of PBMCs, splenocytes or lymphocytes are necessary to collect and prepare.
Rat: Rat is rarely used as an antibody source, but they can be useful at your request.
Nonhuman Primate (rhesus macaque): A special research subject with longer time to induce immune responses. People mostly use this model to mimic human responses, but the cost is high.
Rabbit: Rabbits respond to the broadest classes of antigens with shortest period of time. Antibodies from rabbit may have higher affinity.
Guinea Pig: Guinea pig antibodies are popular choices. They are well-known for their lower backgrounds in drosophila research. But due to the lack of antibody cassettes, we can only supply chimeric antibodies with human Fc, unless you supply guinea pig antibody cassettes. 
Sheep: The large-scale sample source. The main drawback of this option is that sheep take longer to respond and reach maximize antibody titer. Due to the lack of antibody cassettes, we can only supply chimeric antibodies with human Fc, unless you supply sheep antibody cassettes.